This technique utilizes specifically designed RNA molecules to guide the precise integration of genetic material from a donor source into a targeted location within a recipient DNA sequence. This process allows for controlled modification of the genome, facilitating the insertion, deletion, or replacement of genetic elements. For example, a therapeutic gene could be precisely inserted into a non-functional gene within a patient’s cells.
This method offers significant advantages over traditional gene editing approaches. Its programmability enables high specificity, minimizing off-target effects. The direct nature of the recombination process enhances efficiency, potentially streamlining therapeutic development. This emerging technology builds upon earlier RNA-guided gene manipulation techniques and holds promise for advancing gene therapy, disease modeling, and synthetic biology.
